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Mapping Cell Membrane Organization and Dynamics Using Soft Nano-Imprint Lithography

By T. Sansen, D. Sanchez-Fuentes, R. Rathar, Adai Colom, Fatima El Alaoui, Julien Viaud, M. Macchione, S. de Rossi, Stefan Matile, Raphael Gaudin, V. B├Ącker, Adrien Carretero-Genevrier, Laura Picas

Posted 12 Sep 2019
bioRxiv DOI: 10.1101/767590

Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and bench top method allowing to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction. ### Competing Interest Statement The authors have declared no competing interest.

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