Rxivist logo

MADloy: Robust detection of mosaic loss of chromosome Y from genotype-array-intensity data

By Juan R Gonzalez, Marcos López-Sánchez, Alejandro Cáceres, Pere Puig, Tonu Esko, Luis A Pérez-Jurado

Posted 10 Sep 2019
bioRxiv DOI: 10.1101/764845

Accurate protocols and methods to robustly detect the mosaic loss of chromosome Y (mLOY) are needed given its reported role in cancer, several age-related disorders and overall male mortality. Intensity SNP-array data have been used to infer mLOY status and to determine its prominent role in male disease. However, discrepancies of reported findings can be due to the uncertainty and variability of the methods used for mLOY detection and to the differences in the tissue-matrix used. We proposed MADloy , the first publicly available software tool that incorporates previous methods and includes a new robust approach, allowing efficient calling in large studies and comparisons between methods. The new method implemented in MADloy optimizes mLOY calling by correctly modeling the underlying reference population with no-mLOY status and incorporating B-deviation information. We observed improvements in the calling accuracy with respect to previous methods, using experimentally validated samples, and an increment in the statistical power to detect associations with disease and mortality, using simulation studies and real dataset analyses. We applied MADloy to detect the increment of mLOY cellularity in blood on 18 individuals after 3 years, and to confirm that its detection in saliva was sub-optimal (41%). We illustrate the use of MADloy to detect the down-regulation genes in the chromosome Y in kidney and bladder tumors with mLOY, and to perform pathway analyses for the detection of mLOY in blood. MADloy is a new software tool implemented in R for easy and robust calling of mLOY status in men aimed to facilitate its study in large epidemiological studies.

Download data

  • Downloaded 327 times
  • Download rankings, all-time:
    • Site-wide: 79,977
    • In genomics: 5,260
  • Year to date:
    • Site-wide: 66,334
  • Since beginning of last month:
    • Site-wide: 80,169

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News