Rxivist logo

MKPV (aka MuCPV) and related chapparvoviruses are nephro-tropic and encode novel accessory proteins p15 and NS2

By Christopher J Jolly, Quintin Lee, Matthew P. Padula, Natalia Pinello, Simon H. Williams, Matthew B. O’Rourke, Marcilio Jorge Fumagalli, Joseph D Orkin, Babak Shaban, Ori Brenner, Wolfgang Weninger, William Marciel Souza, Amanda D Melin, Justin Wong, Marcus J. Crim, Sébastien Monette, Ben Roediger

Posted 15 Aug 2019
bioRxiv DOI: 10.1101/732537

Mouse kidney parvovirus (MKPV) is a member of the provisional Chapparvovirus genus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four MKPV transcripts, created by alternative splicing, to a common transcription initiation region, and use mass spectrometry to identify p10 and p15 as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and a splicing-dependent putative accessory protein NS2 are conserved in all near-complete tetrapod chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that chapparvoviruses with >60% VP1 amino acid identity to MKPV be classified into a genus dubbed Nephroparvovirus, which is consistent with nomenclature for the genus Erythroparvovirus.

Download data

  • Downloaded 473 times
  • Download rankings, all-time:
    • Site-wide: 70,398
    • In microbiology: 4,633
  • Year to date:
    • Site-wide: 132,754
  • Since beginning of last month:
    • Site-wide: 110,448

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide