Anti-inflammatory role of curcumin in Lipopolysaccharide treated A549 cells at global proteome level and on mycobacterial infection
Rhishikesh R Bargaje,
Mrinal Kumar Das,
Hossain Md. Faruquee,
Rajendra Kumar Behera,
Ranjan Kumar Nanda,
Posted 31 Jul 2019
bioRxiv DOI: 10.1101/721100
Posted 31 Jul 2019
A diet derived agent Curcumin (Diferuloylmethane), demonstrated its clinical application in inflammation, infection and cancer conditions. Nevertheless, its impact on the proteome of epithelial cells of non-small cell lung carcinoma (NSCLC) is yet to be explored. We employed a stable isotope labeling method for cell culture (SILAC) based relative quantitative proteomics and informatics analysis to comprehend global proteome change in A549 cells treated with curcumin and/or Lipopolysaccharide (LPS). Pretreated A549 cells were infected with Mycobacterium tuberculosis H37Rv strain to monitor bacterial load. With exposure to curcumin and LPS, out of the 1492 identified proteins, 305 and 346 proteins showed deregulation respectively. The expression of BID and AIFM1 mitochondrial proteins which play critical role in apoptotic pathway were deregulated in curcumin treated cells. Higher mitochondria intensity was observed in curcumin treated A549 cells than LPS treatment. Simultaneous treatment of curcumin and LPS neutralized the effect of LPS. Curcumin and/or LPS pretreated A549 cells infected with H37Rv, showed successful bacterial internalization. LPS treated A549 cells after infection showed increased bacterial load than curcumin compared to non-treated infected cells. However, simultaneous treatment of curcumin and LPS neutralized the effect of LPS. This study generated molecular evidence to deepen our understanding of the anti-inflammatory role of curcumin and may be useful to identify molecular targets for drug discovery. * NSCLC : non-small cell lung carcinoma SILAC : stable isotope labeling method for cell culture LPS : Lipopolysaccharide Mtb : Mycobacterium tuberculosis BSA : bovine serum albumin PBS : phosphate buffer saline BID : BH3-interacting domain death agonist AIFM1 : apoptosis-inducing factor protein 1 NCCS : National Centre for Cell Science DAPI : 4′,6-diamidino-2-phenylindole TLRs : Toll-like receptors TNF-α : Tumor necrosis factor α GAPDH : Glyceraldehyde 3-phosphate dehydrogenase
- Downloaded 309 times
- Download rankings, all-time:
- Site-wide: 55,099 out of 100,173
- In biochemistry: 1,681 out of 3,421
- Year to date:
- Site-wide: 23,059 out of 100,173
- Since beginning of last month:
- Site-wide: 42,309 out of 100,173
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!