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Noninvasive Prenatal Diagnosis for Duchenne Muscular Dystrophy Based on the Direct Haplotype Phasing

By Min Chen, Chao Chen, Xiaoyan Huang, Jun Sun, Lu Jiang, Yingting Li, Yaping Zhu, Changgeng Tian, Yufan Li, Zhe Lu, Yaoshen Wang, Fanwei Zeng, Yun Yang, Zhiyu Peng, Chenghong Yin, Dunjin Chen

Posted 31 Jul 2019
bioRxiv DOI: 10.1101/720987 (published DOI: 10.1002/pd.5641)

Objective: We aimed to investigate the validity of noninvasive prenatal diagnosis (NIPD) based on direct haplotype phasing without the proband and its feasibility for clinical application in the case of Duchenne Muscular Dystrophy (DMD). Methods: Thirteen singleton-pregnancy families affected by DMD were recruited. Firstly, we resolved maternal haplotypes for each family by performing targeted linked-read sequencing of their high molecular weight DNA, respectively. Then, we identified SNPs of the DMD gene in all carrier mothers and inferred the DMD gene mutation status of all fetuses. Finally, the fetal genotypes were further validated by using chorionic villus sampling. Results: The method of directly resolving maternal haplotype through targeted linked-read sequencing was smoothly performed in all participated families. The predicted mutational status of 13 fetuses was correct, which had been confirmed by invasive prenatal diagnosis. Conclusion: Direct haplotyping of NIPD based on linked-read sequencing for DMD is accurate.

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