Rxivist logo

A 6-nm ultra-photostable DNA Fluorocube for fluorescence imaging

By Stefan Niekamp, Nico Stuurman, Ronald D. Vale

Posted 27 Jul 2019
bioRxiv DOI: 10.1101/716787 (published DOI: 10.1038/s41592-020-0782-3)

Photobleaching limits extended imaging of fluorescent biological samples. Here, we developed DNA origami-based Fluorocubes that are similar in size to the green fluorescent protein (GFP), have single-point attachment to proteins, have a 50-fold higher photobleaching lifetime and emit 40-fold more photons than single organic dyes. We demonstrate that DNA Fluorocubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.

Download data

  • Downloaded 3,077 times
  • Download rankings, all-time:
    • Site-wide: 1,601 out of 83,873
    • In biophysics: 32 out of 3,595
  • Year to date:
    • Site-wide: 2,388 out of 83,873
  • Since beginning of last month:
    • Site-wide: 9,488 out of 83,873

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)