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A 6-nm ultra-photostable DNA Fluorocube for fluorescence imaging

By Stefan Niekamp, Nico Stuurman, Ronald D. Vale

Posted 27 Jul 2019
bioRxiv DOI: 10.1101/716787 (published DOI: 10.1038/s41592-020-0782-3)

Photobleaching limits extended imaging of fluorescent biological samples. Here, we developed DNA origami-based Fluorocubes that are similar in size to the green fluorescent protein (GFP), have single-point attachment to proteins, have a 50-fold higher photobleaching lifetime and emit 40-fold more photons than single organic dyes. We demonstrate that DNA Fluorocubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.

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