Targeting DNA damage response and Replication Stress in Pancreatic Cancer
Stephan B. Dreyer,
Derek W. Wright,
Colin J. McKay,
Grant A. McGregor,
Michele L. Dziubinski,
Simon T. Barry,
Glasgow Precision Oncology Laboratory,
Australian Pancreatic Cancer Genome Initiative,
Fieke E. M. Froeling,
Elizabeth A. Musgrove,
Gloria M. Petersen,
Margaret C. Frame,
Howard C. Crawford,
Diane M. Simeone,
Susanna L Cooke,
Nigel B. Jamieson,
Jennifer P Morton,
Owen J. Sansom,
Peter J. Bailey,
Andrew V. Biankin,
David K Chang
Posted 24 Jul 2019
bioRxiv DOI: 10.1101/713545
Posted 24 Jul 2019
Continuing recalcitrance to therapy cements pancreatic cancer (PC) as the most lethal malignancy, which is set to become the second leading cause of cancer death in our society. We interrogated the transcriptome, genome, proteome and functional characteristics of 61 novel PC patient-derived cell lines to define novel therapeutic strategies targeting the DNA damage response (DDR) and replication stress. We show that patient-derived cell lines faithfully recapitulate the epithelial component of pancreatic tumors including previously described molecular subtypes. Biomarkers of DDR deficiency, including a novel signature of homologous recombination deficiency, co-segregates with response to platinum and PARP inhibitor therapy in vitro and in vivo. We generated a novel signature of replication stress with potential clinical utility in predicting response to ATR and WEE1 inhibitor treatment. Replication stress and DDR deficiency are independent of each other, creating opportunities for therapy in DDR proficient PC, and post-platinum therapy.
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