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Wide-field time-gated SPAD imager for phasor-based FLIM applications

By Arin Ulku, Andrei Ardelean, Michel Antolovic, Shimon Weiss, Edoardo Charbon, Claudio Bruschini, Xavier Michalet

Posted 30 Jun 2019
bioRxiv DOI: 10.1101/687277 (published DOI: 10.1088/2050-6120/ab6ed7)

We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512×512 pixels), even in the case of small number of gates and limited photon budget.

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