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Chemogenetic control of nanobodies

By Helen Farrants, Miroslaw Tarnawski, Thorsten G. Mueller, Shotaro Otsuka, Julien Hiblot, Birgit Koch, Moritz Kueblbeck, Hans-Georg Kraeusslich, Jan Ellenberg, Kai Johnsson

Posted 27 Jun 2019
bioRxiv DOI: 10.1101/683557 (published DOI: 10.1038/s41592-020-0746-7)

We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.

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