Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,793 bioRxiv papers from 266,023 authors.

A mechanical model reveals that non-axisymmetric buckling lowers the energy barrier associated with membrane neck constriction

By Ritvik Vasan, Shiva Rudraraju, Matthew Akamatsu, Krishna Garikipati, Padmini Rangamani

Posted 15 Jun 2019
bioRxiv DOI: 10.1101/672485

Membrane neck formation is essential for scission, which, as recent experiments on tubules have demonstrated, can be location dependent. The diversity of biological machinery that can constrict a neck such as dynamin, actin, ESCRTs and BAR proteins, and the range of forces and deflection over which they operate, suggest that the constriction process is functionally mechanical and robust to changes in biological environment. In this study, we used a mechanical model of the lipid bilayer to systematically investigate the influence of location, symmetry constraints, and helical forces on membrane neck constriction. Simulations from our model demonstrated that the energy barriers associated with constriction of a membrane neck are location-dependent. Importantly, if symmetry restrictions are relaxed, then the energy barrier for constriction is dramatically lowered and the membrane buckles at lower values of forcing parameters. Our simulations also show that constriction due to helical proteins further reduces the energy barrier for neck formation compared to cylindrical proteins. These studies establish that despite different molecular mechanisms of neck formation in cells, the mechanics of constriction naturally leads to a loss of symmetry that can lower the energy barrier to constriction.

Download data

  • Downloaded 258 times
  • Download rankings, all-time:
    • Site-wide: 34,061 out of 57,793
    • In biophysics: 1,364 out of 2,450
  • Year to date:
    • Site-wide: 10,709 out of 57,793
  • Since beginning of last month:
    • Site-wide: 11,198 out of 57,793

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)