Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 59,974 bioRxiv papers from 266,630 authors.

Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth

By Erin N Howe, Miranda D. Burnette, Melanie E. Justice, James W. Clancy, Ian H. Guldner, Patricia M. Schnepp, Victoria Hendrick, Uma K Aryal, Alicia T. Specht, Jun Li, Crislyn D'Souza-Schorey, Jeremiah James Zartman, Siyuan Zhang

Posted 11 Jun 2019
bioRxiv DOI: 10.1101/666750

Breast cancer brain metastases (BCBM) have a 5-20 year latency and account for up to 30% of mortality. Developing new therapeutics requires a molecular understanding of adaptation to the brain microenvironment. Here, we combined RNA-sequencing of BCBM development with a reverse genetic screen in Drosophila melanogaster and identified Rab11b, an endosomal recycling protein, as a mediator of metastatic adaptation. We show that disseminated cells up-regulated Rab11b early after arrival in the brain, allowing control of the cell surface proteome through recycling of proteins required for successful interaction with the microenvironment, including integrin β1. Rab11b-mediated control of integrin β1 surface expression allows ligation to the brain ECM, activating mechanotransduction signaling to allow survival and proliferation. We propose a model in which up-regulation of Rab11b allows disseminated cells to recycle needed proteins during metastatic adaptation, without strictly requiring transcription and translation, to allow for metastatic outgrowth.

Download data

  • Downloaded 198 times
  • Download rankings, all-time:
    • Site-wide: 42,371 out of 59,974
    • In cancer biology: 1,334 out of 1,964
  • Year to date:
    • Site-wide: 17,812 out of 59,974
  • Since beginning of last month:
    • Site-wide: 16,117 out of 59,974

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)