For nanosecond pulsed electric fields (nsPEFs) based application, the underlying transmembrane potential (TMP) distribution of the plasma membrane is influenced by electroporation (EP) of the plasma membrane and dispersion (DP) of all cell compartments and is important for predicting the bioelectric effects. In this study, we analysed temporal and spatial distribution of TMP induced by nsPEFs of various durations (3 ns, 5 ns unipolar, 5 ns bipolar, and 10 ns) with the consideration of both DP and EP. Based on the double-shelled dielectric spherical cell model, we used second-order Debye equation to characterize the dielectric relaxation of plasma membrane and nuclear membrane in the frequency domain and transformed the Debye equation into the time domain with the introduction of polarization vector, then we obtained the time course of TMP by solving the combination of Laplace equation and time-domain Debye equation. Next, we used the asymptotic version of the smoluchowski equation to characterize electroporation of plasma membrane and added it to our model to achieve the temporal and spatial distribution of TMP and pore density. Much faster and more pronounced increased in TMP can be found with the consideration of dielectric relaxation of plasma membrane and nuclear membrane, and much larger electroporated area of at least half of the plasma membrane was obtained with the consideration of both DP and EP. Through the simulation it is clearer to understand the relationship.
- Downloaded 280 times
- Download rankings, all-time:
- Site-wide: 99,672
- In bioengineering: 1,909
- Year to date:
- Site-wide: 144,277
- Since beginning of last month:
- Site-wide: 135,582
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!