Rxivist logo

Transient transcriptome sequencing: computational pipeline to quantify genome-wide RNA kinetic parameters and transcriptional enhancer activity

By Gabriel Villamil, Leonhard Wachutka, Patrick Cramer, Julien Gagneur, Björn Schwalb

Posted 04 Jun 2019
bioRxiv DOI: 10.1101/659912

In the accompanying chapter (Gressel, Lidschreiber, Cramer), we describe the detailed experimental protocol for transient transcriptome sequencing (TT-seq). TT-seq detects metabolically labeled, newly synthesized RNA fragments genome-wide in living cells. TT-seq can monitor gene activity and the dynamics of enhancer landscapes with great sensitivity, but this requires careful computational analysis of the data. In this manuscript, we present the bioinformatics workflow used to analyze TT-seq data. In particular, we describe pre-processing steps, including a reliable and robust normalization strategy, and several downstream analysis tools that enable the user to quantify RNA synthesis, splicing and degradation activities. Together, these tools form a comprehensive analysis pipeline that can be adapted to almost any TT-seq application.

Download data

  • Downloaded 1,151 times
  • Download rankings, all-time:
    • Site-wide: 19,509
    • In molecular biology: 527
  • Year to date:
    • Site-wide: 20,028
  • Since beginning of last month:
    • Site-wide: 28,219

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide