General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM

Despite their great potential to facilitate rapid preparation of quite impure samples, affinity grids have not yet been widely employed in single particle cryo-EM. Here, we chemically functionalize graphene oxide coated grids and use a highly specific covalent affinity tag system. Importantly, our polyethylene glycol spacer keeps particles away from the air-water interface and graphene oxide surface, protecting them from denaturation or aggregation and permits high-resolution reconstructions of small particles.

Download data

• Downloaded 2,051 times
• Download rankings, all-time:
  • Site-wide: 6,678
  • In biophysics: 133
• Year to date:
  • Site-wide: 30,318
• Since beginning of last month:
  • Site-wide: 30,318

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide