Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,177 bioRxiv papers from 306,444 authors.
High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange
Sarah A Overall,
Jugmohit S Toor,
Alberto S Japp,
Michael R Betts,
John M. Maris,
Nikolaos G. Sgourakis
Posted 30 May 2019
bioRxiv DOI: 10.1101/653477
Posted 30 May 2019
Peptide exchange technologies are essential for the generation of pMHC-multimer libraries, used to probe highly diverse, polyclonal TCR repertoires. Using the molecular chaperone TAPBPR, we present a robust method for the capture of stable, empty MHC-I molecules which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. Combined with tetramer barcoding using multi-modal cellular indexing technology (ECCITE-seq), our approach allows a combined analysis of TCR repertoires and other T-cell transcription profiles together with their cognate pMHC-I specificities in a single experiment.
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