Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 63,093 bioRxiv papers from 279,869 authors.
High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange
Sarah A Overall,
Jugmohit S Toor,
Alberto S Japp,
Michael R Betts,
John M. Maris,
Nikolaos G Sgourakis
Posted 30 May 2019
bioRxiv DOI: 10.1101/653477
Posted 30 May 2019
Peptide exchange technologies are essential for the generation of pMHC-multimer libraries, used to probe highly diverse, polyclonal TCR repertoires. Using the molecular chaperone TAPBPR, we present a robust method for the capture of stable, empty MHC-I molecules which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. Combined with tetramer barcoding using multi-modal cellular indexing technology (ECCITE-seq), our approach allows a combined analysis of TCR repertoires and other T-cell transcription profiles together with their cognate pMHC-I specificities in a single experiment.
- Downloaded 591 times
- Download rankings, all-time:
- Site-wide: 15,507 out of 63,093
- In immunology: 266 out of 1,571
- Year to date:
- Site-wide: 3,736 out of 63,093
- Since beginning of last month:
- Site-wide: 7,888 out of 63,093
Downloads over time
Distribution of downloads per paper, site-wide
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!