Rxivist logo

Fate mapping via Ms4a3 expression history traces monocyte-derived cells

By Zhaoyuan Liu, Yaqi Gu, Svetoslav Chakarov, Camille Bleriot, Xin Chen, Amanda Shin, Weijie Huang, Regine J. Dress, Charles-Antoine Dutertre, Andreas Schlitzer, Jinmiao Chen, Honglin Wang, Zhiduo Liu, Bing Su, Florent GINHOUX

Posted 27 May 2019
bioRxiv DOI: 10.1101/652032 (published DOI: 10.1016/j.cell.2019.08.009)

Most tissue-resident macrophage (RTM) populations are seeded by waves of embryonic hematopoiesis and are self-maintained independently of a bone-marrow contribution during adulthood. A proportion of RTMs, however, is constantly replaced by blood monocytes and their functions compared to embryonic RTM remains unclear. The kinetics and extent of the contribution of circulating monocytes to RTM replacement during homeostasis, inflammation and disease is highly debated. Here, we identified Ms4a3 as a specific marker expressed by granulocyte-monocyte progenitors (GMPs) and subsequently generated Ms4a3TdT reporter and Ms4a3Cre-RosaTdT fate mapper models to follow monocytes and their progenies. Our Ms4a3Cre-RosaTdT model traced efficiently blood monocytes (97%) and granulocytes (100%), but no lymphocytes or tissue dendritic cells. Using this model, we precisely quantified the contribution of monocytes to the RTM pool during homeostasis and inflammation. The unambiguous identification of monocyte-derived cells will permit future studies of their function under any condition.

Download data

  • Downloaded 3,472 times
  • Download rankings, all-time:
    • Site-wide: 4,708
    • In immunology: 175
  • Year to date:
    • Site-wide: 24,441
  • Since beginning of last month:
    • Site-wide: 66,967

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide