Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 73,481 bioRxiv papers from 319,823 authors.

Enabling high-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing

By Søeren M. Karst, Ryan M Ziels, Rasmus Hansen Kirkegaard, Emil A. Sørensen, Daniel McDonald, Qiyun Zhu, Rob Knight, Mads Albertsen

Posted 24 May 2019
bioRxiv DOI: 10.1101/645903

High-throughput amplicon sequencing of large genomic regions remains challenging for short-read technologies. Here, we report a high-throughput amplicon sequencing approach combining unique molecular identifiers (UMIs) with Oxford Nanopore Technologies or Pacific Biosciences CCS sequencing, yielding high accuracy single-molecule consensus sequences of large genomic regions. Our approach generates amplicon and genomic sequences of >10,000 bp in length with a mean error-rate of 0.0049-0.0006% and chimera rate <0.022%.

Download data

  • Downloaded 5,420 times
  • Download rankings, all-time:
    • Site-wide: 430 out of 73,481
    • In molecular biology: 10 out of 2,393
  • Year to date:
    • Site-wide: 54 out of 73,481
  • Since beginning of last month:
    • Site-wide: 54 out of 73,481

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News