High-throughput amplicon sequencing of large genomic regions remains challenging for short-read technologies. Here, we report a high-throughput amplicon sequencing approach combining unique molecular identifiers (UMIs) with Oxford Nanopore Technologies or Pacific Biosciences CCS sequencing, yielding high accuracy single-molecule consensus sequences of large genomic regions. Our approach generates amplicon and genomic sequences of >10,000 bp in length with a mean error-rate of 0.0049-0.0006% and chimera rate <0.022%.

Download data

• Downloaded 10,963 times
• Download rankings, all-time:
  • Site-wide: 556 out of 118,150
  • In molecular biology: 10 out of 3,617
• Year to date:
  • Site-wide: 644 out of 118,150
• Since beginning of last month:
  • Site-wide: 559 out of 118,150

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide