Enabling high-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing
By
Søren M Karst,
Ryan M. Ziels,
Rasmus H Kirkegaard,
Emil A. Sørensen,
Daniel McDonald,
Qiyun Zhu,
Rob Knight,
Mads Albertsen
Posted 24 May 2019
bioRxiv DOI: 10.1101/645903
High-throughput amplicon sequencing of large genomic regions remains challenging for short-read technologies. Here, we report a high-throughput amplicon sequencing approach combining unique molecular identifiers (UMIs) with Oxford Nanopore Technologies or Pacific Biosciences CCS sequencing, yielding high accuracy single-molecule consensus sequences of large genomic regions. Our approach generates amplicon and genomic sequences of >10,000 bp in length with a mean error-rate of 0.0049-0.0006% and chimera rate <0.022%.
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