Rxivist logo

Dynamic DNA structure states interact with the RNA editing enzyme ADAR1 to modulate fear extinction memory

By Paul R. Marshall, Qiongyi Zhao, Xiang Li, Wei Wei, Abi Malathi, Esmi Zajaczkowski, Laura Leighton, Sachithrani Madugalle, Dean Basic, Ziqi Wang, Jiayu Yin, Wei-Siang Liau, Carl Walkley, Timothy W Bredy

Posted 20 May 2019
bioRxiv DOI: 10.1101/641209

RNA modification has recently emerged as an important mechanism underlying gene diversity linked to behavioral regulation. The conversion of adenosine to inosine by the ADAR family of enzymes is a particularly important RNA modification as it impacts the physiological readout of protein-coding genes. However, not all variants of ADAR appear to act solely on RNA. ADAR1 binds directly to DNA when it is in a non-canonical, left handed, Z conformation, but little is known about the functional relevance of this interaction. Here we report that ADAR1 binds to Z-DNA in an activity-dependent manner and that fear extinction learning leads to increased ADAR1 occupancy at DNA repetitive elements, with targets adopting a Z-DNA structure at sites of ADAR1 recruitment. Knockdown of ADAR1 leads to an inability to modify a previously acquired memory trace and this is associated with a concomitant change in DNA structure and a decrease in RNA editing. These findings suggest a novel mechanism of learning-induced gene regulation whereby ADAR1 physically interacts with Z-DNA in order to mediate its effect on RNA, and both are required for memory flexibility following fear extinction learning.

Download data

  • Downloaded 711 times
  • Download rankings, all-time:
    • Site-wide: 48,470
    • In neuroscience: 6,690
  • Year to date:
    • Site-wide: 17,849
  • Since beginning of last month:
    • Site-wide: 104,262

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News