Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,910 bioRxiv papers from 266,458 authors.
Protein and small molecule-based CRISPR inhibitors have been developed as selective off-switches to modulate CRISPR-Cas activity. These anti-CRISPR agents have important therapeutic implications because of their capability to improve the specificity of CRISPR-Cas in human cells. In this study, we reported the discovery of Cas9-inactivating peptides from inoviridae bacteriophages. These peptides, derived from the periplasmic domain of phage major coat protein G8P (G8PPD), can inhibit the in vitro cleavage activity of purified Streptococcus pyogenes Cas9 (SpyCas9) proteins in an allosteric manner. Ectopic expression of full-length G8P (G8PFL) or G8PPD in human cells can inactivate the genome-editing activity of SpyCas9 with minimum alterations of the mutation patterns. Most importantly, timed delivery of G8PPD can reduce the off-target activity of co-transfected SpyCas9 without compromising its on-target activity. Therefore, the G8Ps discovered in the current study represent the first anti-CRISPR peptides that can improve the specificity of constitutively expressed SpyCas9, implying the potential applications of G8Ps for precision genome editing.
- Downloaded 278 times
- Download rankings, all-time:
- Site-wide: 32,106 out of 57,910
- In genomics: 3,034 out of 4,058
- Year to date:
- Site-wide: 9,582 out of 57,910
- Since beginning of last month:
- Site-wide: 11,291 out of 57,910
Downloads over time
Distribution of downloads per paper, site-wide
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!