Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,230 bioRxiv papers from 306,637 authors.

Resolving the 3D landscape of transcription-linked mammalian chromatin folding

By Tsung-Han S. Hsieh, Elena Slobodyanyuk, Anders S Hansen, Claudia Cattoglio, Oliver J. Rando, Robert Tjian, Xavier Darzacq

Posted 17 May 2019
bioRxiv DOI: 10.1101/638775

Chromatin folding below the scale of topologically associating domains (TADs) remains largely unexplored in mammals. Here, we used a high-resolution 3C-based method, Micro-C, to probe links between 3D-genome organization and transcriptional regulation in mouse stem cells. Combinatorial binding of transcription factors, cofactors, and chromatin modifiers spatially segregate TAD regions into "microTADs" with distinct regulatory features. Enhancer-promoter and promoter-promoter interactions extending from the edge of these domains predominantly link co-regulated loci, often independently of CTCF/Cohesin. Acute inhibition of transcription disrupts the gene-related folding features without altering higher-order chromatin structures. Intriguingly, we detect "two-start" zig-zag 30-nanometer chromatin fibers. Our work uncovers the finer-scale genome organization that establishes novel functional links between chromatin folding and gene regulation.

Download data

  • Downloaded 3,718 times
  • Download rankings, all-time:
    • Site-wide: 821 out of 70,230
    • In genomics: 209 out of 4,700
  • Year to date:
    • Site-wide: 1,129 out of 70,230
  • Since beginning of last month:
    • Site-wide: 297 out of 70,230

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)