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Assessment of single cell RNA-seq normalization methods

By Bo Ding, Lina Zheng, Wei Wang

Posted 17 Jul 2016
bioRxiv DOI: 10.1101/064329 (published DOI: 10.1534/g3.117.040683)

We have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Our analyses showed that methods considering spike-in ERCC RNA molecules significantly outperformed those not considering ERCCs. This work provides a guidance of selecting normalization methods to remove technical noise in single cell RNA-seq data.

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