Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,132 bioRxiv papers from 295,346 authors.

The photoactive site modulates current rectification and channel closing in the natural anion channelrhodopsin GtACR1

By Oleg A Sineshchekov, Elena G Govorunova, Hai Li, Xin Wang, John L Spudich

Posted 07 May 2019
bioRxiv DOI: 10.1101/630327

The crystal structure of Gt ACR1 from Guillardia theta revealed an intramolecular tunnel predicted to expand to form the anion-conducting channel upon photoactivation (Li et al. 2019). The location of the retinylidene photoactive site within the tunnel raised the question of whether, in addition to triggering channel opening by photoisomerization, the site also participates in later channel processes. Here we demonstrate the involvement of the photoactive site in chloride conductance and channel closing. Electrostatic perturbation of the photoactive retinylidene Schiff base region by glutamate substitutions alters the rectification of the photocurrent as well as channel closing kinetics. Substitutions on opposite sides of the photoactive site causes opposite changes, with channel closing kinetically correlated with Schiff base deprotonation, and the extent of these effects closely correlate with distance of the introduced glutamyl residue from the photoactive site.

Download data

  • Downloaded 194 times
  • Download rankings, all-time:
    • Site-wide: 49,084 out of 67,143
    • In biochemistry: 1,413 out of 1,974
  • Year to date:
    • Site-wide: 26,119 out of 67,143
  • Since beginning of last month:
    • Site-wide: 20,517 out of 67,143

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News