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Photoactivation of silicon rhodamines via a light-induced protonation

By Michelle S. Frei, Philipp Hoess, Marko Lampe, Bianca Nijmeijer, Moritz Kueblbeck, Jan Ellenberg, Jonas Ries, Stefan Pitsch, Luc Reymond, Kai Johnsson

Posted 04 May 2019
bioRxiv DOI: 10.1101/626853 (published DOI: 10.1038/s41467-019-12480-3)

We present a new type of photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent isomerization followed by protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we created probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore's outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

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