Rxivist logo

Photoactivation of silicon rhodamines via a light-induced protonation

By Michelle S. Frei, Philipp Hoess, Marko Lampe, Bianca Nijmeijer, Moritz Kueblbeck, Jan Ellenberg, Jonas Ries, Stefan Pitsch, Luc Reymond, Kai Johnsson

Posted 04 May 2019
bioRxiv DOI: 10.1101/626853 (published DOI: 10.1038/s41467-019-12480-3)

We present a new type of photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent isomerization followed by protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we created probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore's outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

Download data

  • Downloaded 760 times
  • Download rankings, all-time:
    • Site-wide: 14,345 out of 77,361
    • In biophysics: 462 out of 3,291
  • Year to date:
    • Site-wide: 47,419 out of 77,361
  • Since beginning of last month:
    • Site-wide: 47,230 out of 77,361

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)