Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,836 bioRxiv papers from 309,140 authors.
Photoactivation of silicon rhodamines via a light-induced protonation
Michelle S. Frei,
Posted 04 May 2019
bioRxiv DOI: 10.1101/626853 (published DOI: 10.1038/s41467-019-12480-3)
Posted 04 May 2019
We present a new type of photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent isomerization followed by protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we created probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore's outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.
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