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A comprehensive plasma metabolomics dataset for a cohort of mouse knockouts within the international mouse phenotyping consortium

By Dinesh K Barupal, Ying Zhang, Tong Shen, Sili Fan, Bryan S Roberts, Patrick Fitzgerald, Benjamin Wancewicz, Luis Valdiviez, Gert Wohlgemuth, Gregory Byram, YingYng Choy, Bennett Haffner, Megan R Showalter, Arpana Vaniya, Clayton S Bloszies, Jacob S. Folz, Tobias Kind, Oliver Fiehn

Posted 02 May 2019
bioRxiv DOI: 10.1101/624437 (published DOI: 10.3390/metabo9050101)

Mouse knockouts allow studying gene functions. Often, multiple phenotypes are impacted when a gene is inactivated. The International Mouse Phenotyping Consortium (IPMC) has generated thousands of mouse knockouts and catalogued their phenotype data. We have acquired metabolomics data from 220 plasma samples of 30 mouse gene knockouts and corresponding wildtype mice from IMPC. To acquire comprehensive metabolomics data, we have used liquid chromatography (LC) combined with mass spectrometry (MS) for detecting polar and lipophilic compounds in an untargeted approach. We have also used targeted methods to measure bile acids, steroids and oxylipins. In addition, we have used gas chromatography GC-TOFMS for measuring primary metabolites. The metabolomics dataset reports 832 unique structurally identified metabolites from 124 chemical classes as determined by ChemRICH software. The GCMS and LCMS raw data files, intermediate and finalized data matrices, R-Scripts, annotation databases and extracted ion chromatograms are provided in this data descriptor. The dataset can be used for subsequent studies to link genetic variants with molecular mechanisms and phenotypes.

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