Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 60,207 bioRxiv papers from 267,649 authors.

Critical length in long read resequencing

By Wouter De Coster, Mojca Strazisar, Peter De Rijk

Posted 29 Apr 2019
bioRxiv DOI: 10.1101/621862

Long read sequencing has a substantial advantage for structural variant discovery and phasing of variants compared to short-read technologies, but the required and optimal read length has not been assessed. In this work, we used simulated long reads and evaluated structural variant discovery and variant phasing using current best practice bioinformatics methods. We determined that optimal discovery of structural variants from human genomes can be obtained with reads of minimally 15 kbp. Haplotyping genes entirely only reaches its optimum from reads of 100 kbp. These findings are important for the design of future long read sequencing projects.

Download data

  • Downloaded 420 times
  • Download rankings, all-time:
    • Site-wide: 22,528 out of 60,207
    • In bioinformatics: 3,210 out of 6,077
  • Year to date:
    • Site-wide: 5,708 out of 60,207
  • Since beginning of last month:
    • Site-wide: 22,682 out of 60,207

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News