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All-optical electrophysiology reveals excitation, inhibition, and neuromodulation in cortical layer 1

By Linlin Z. Fan, Simon Kheifets, Urs L. Boehm, Kiryl D. Piatkevich, Hao Wu, Vicente Parot, Michael Xie, Edward S Boyden, Anne E. Takesian, Adam E Cohen

Posted 23 Apr 2019
bioRxiv DOI: 10.1101/614172

The stability of neural dynamics arises through a tight coupling of excitatory (E) and inhibitory (I) signals. Genetically encoded voltage indicators (GEVIs) can report both spikes and subthreshold dynamics in vivo, but voltage only reveals the combined effects of E and I synaptic inputs, not their separate contributions individually. Here we combine optical recording of membrane voltage with simultaneous optogenetic manipulation to probe E and I individually in barrel cortex Layer 1 (L1) neurons in awake mice. Our studies reveal how the L1 microcircuit integrates thalamocortical excitation, lateral inhibition and top-down neuromodulatory inputs. We develop a simple computational model of the L1 microcircuit which captures the main features of our data. Together, these results suggest a model for computation in L1 interneurons consistent with their hypothesized role in attentional gating of the underlying cortex. Our results demonstrate that all-optical electrophysiology can reveal basic principles of neural circuit function in vivo.

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