Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,317 bioRxiv papers from 307,048 authors.

PSC-RED, an Albumin-Free Robust Erythroid Differentiation Method to Produce Enucleated Red Blood Cells from Human Pluripotent Stem Cells

By Emmanuel N Olivier, Shouping Zhang, Zi Yan, Sandra Suzuka, Karl Roberts, Kai Wang, Eric E Bouhassira

Posted 23 Apr 2019
bioRxiv DOI: 10.1101/616748

Cultured red blood cells (cRBCs) have many potential applications in transfusion medicine and drug delivery. We report that we have developed chemically defined, albumin-free Robust Erythroid Differentiation (RED) methods to produce enucleated cRBCs from human induced pluripotent stem cells (iPSCs). Human iPSC-derived cRBCs produced with either the short or long variation of the RED protocol respectively express embryonic/fetal or a mixture of fetal and adult hemoglobins. The long version of the protocol produces up to 50% of enucleated cells at an unprecedented yield. RED is scalable and relies on inexpensive components and therefore dramatically increases the feasibility and economic viability of all translational applications of cRBCs.

Download data

  • Downloaded 275 times
  • Download rankings, all-time:
    • Site-wide: 41,486 out of 70,317
    • In bioengineering: 756 out of 1,435
  • Year to date:
    • Site-wide: 9,037 out of 70,317
  • Since beginning of last month:
    • Site-wide: 16,750 out of 70,317

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)