Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,588 bioRxiv papers from 298,004 authors.

In Vitro Potent Activity of ε-poly-L-lysine against Candida albicans and the Underlying Mechanisms

By Lian-hua Wei, Tian Yu, Xiao-ning Wang, Jin-xia Hou, Xin Wang, Chuan Wang, Ke-ke Li, Shuang-yan Jing, Xu Zhang

Posted 12 Apr 2019
bioRxiv DOI: 10.1101/605857

Objective: This study aimed to examine the antifungal activity of e-poly-L-lysine (e-PL) against the planktonic cells or biofilms of Candida albicans and explore the underlying mechanism. Methods: The minimal inhibitory concentration, minimum fungal concentration, and sessile minimal inhibitory concentration were estimated. The germ tube formation and yeast-to-hypha transformation of C. albicans in different media that induced mycelial growth were recorded. The effect of different concentrations of e-PL on the biofilm formation process and mature biofilm of C. albicans was determined. The reactive oxygen species (ROS) and malondialdehyde (MDA) contents of C. albicans after e-PL treatment were measured. The changes in major virulence genes and proteins of C. albicans were detected. Results: e-PL (512 mg/mL) exerted a strong inhibitory effect on C. albicans and biofilms. It blocked the yeast-to-hypha transition and reduced the germ tube formation and germ tube length of C. albicans. The MDA and ROS contents showed an upward trend, indicating a positive correlation with the concentration. Further, e-PL inhibited the high expression of virulence genes in oxidative stress induced by C. albicans. The main peak in the mass spectrum of C. albicans was found to be clear. Conclusions: e-PL exerted a significant antifungal effect on the phytoplankton and biofilm of C. albicans. High concentrations of e-PL significantly inhibited the main mycelium of C. albicans. e-PL induced ROS, released cytochrome C, attacked the C. albicans cell membrane to aggravate its lipid oxidation, and inhibited the expression of C. albicans-associated virulence genes and proteins, thereby exerting a bacteriostatic effect.

Download data

  • Downloaded 158 times
  • Download rankings, all-time:
    • Site-wide: 54,683 out of 67,590
    • In microbiology: 4,007 out of 5,468
  • Year to date:
    • Site-wide: 33,659 out of 67,590
  • Since beginning of last month:
    • Site-wide: 56,121 out of 67,590

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)