Rxivist logo

The bacterial leader peptide peTrpL has a conserved function in antibiotic-dependent posttranscriptional regulation of ribosomal genes

By Hendrik Melior, Sandra Maaß, Maximilian Stötzel, Siqi Li, Konrad U. Förstner, Rubina Schütz, Saina Azarderakhsh, Aleksei Shevkoplias, Susanne Barth-Weber, Adithi R. Varadarajan, Kathrin Baumgardt, Zoe Chervontseva, John Ziebuhr, Christian H. Ahrens, Dörte Becher, Elena Evguenieva-Hackenberg

Posted 12 Apr 2019
bioRxiv DOI: 10.1101/606483

The ribosome-dependent attenuator located upstream of bacterial tryptophan biosynthesis genes harbors a small ORF trpL containing tryptophan codons. When tryptophan is available, efficient trpL translation causes transcription termination and release of the attenuator RNA rnTrpL. In Sinorhizobium meliloti , rnTrpL is a trans -acting sRNA. Here, we identified an evolutionary conserved function for the trpL -encoded 14-aa leader peptide peTrpL. Upon exposure to tetracycline, the cellular peTrpL levels were increased and rnTrpL was generated independently of tryptophan availability. Both peTrpL and rnTrpL were found to be involved in tetracycline-dependent destabilization of rplUrpmA mRNA encoding ribosomal proteins L21 and L27. We provide evidence for redirection of the sRNA rnTrpL from its antibiotic-independent target trpDC to rplUrpmA by formation of an antibiotic-dependent ribonucleoprotein complex (ARNP). ARNPs comprising peTrpL, rnTrpL, rplUrpmA and antisense RNA were also observed for other translation-inhibiting antibiotics, suggesting that bacteria evolved mechanisms to utilize antibiotics for mRNA destabilization.

Download data

  • Downloaded 326 times
  • Download rankings, all-time:
    • Site-wide: 49,017 out of 88,403
    • In microbiology: 3,540 out of 7,608
  • Year to date:
    • Site-wide: 27,790 out of 88,403
  • Since beginning of last month:
    • Site-wide: 42,876 out of 88,403

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News