Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,316 bioRxiv papers from 296,340 authors.

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

By Daniel J Nieves, Geva Hilzenrat, Jason Tran, Zhengmin Yang, Hugh H. MacRae, Matthew AB Baker, John Justin Gooding, Katharina Gaus

Posted 10 Apr 2019
bioRxiv DOI: 10.1101/604462

Recently, DNA-PAINT single molecule localisation microscopy (SMLM) has shown great promise for quantitative imaging. However, labelling strategies so far have relied on approaches that are multivalent or affinity-based. Here, we demonstrate tagPAINT - the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA docking strands for single molecule localisation microscopy via DNA-PAINT. We utilised tagPAINT for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

Download data

  • Downloaded 546 times
  • Download rankings, all-time:
    • Site-wide: 18,918 out of 67,316
    • In biophysics: 663 out of 2,854
  • Year to date:
    • Site-wide: 5,593 out of 67,316
  • Since beginning of last month:
    • Site-wide: 10,460 out of 67,316

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)