Rxivist logo

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

By Daniel J. Nieves, Geva Hilzenrat, Jason Tran, Zhengmin Yang, Hugh H. MacRae, Matthew AB Baker, J Justin Gooding, Katharina Gaus

Posted 10 Apr 2019
bioRxiv DOI: 10.1101/604462 (published DOI: 10.1098/rsos.191268)

Recently, DNA-PAINT single molecule localisation microscopy (SMLM) has shown great promise for quantitative imaging. However, labelling strategies so far have relied on approaches that are multivalent or affinity-based. Here, we demonstrate tagPAINT - the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA docking strands for single molecule localisation microscopy via DNA-PAINT. We utilised tagPAINT for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

Download data

  • Downloaded 689 times
  • Download rankings, all-time:
    • Site-wide: 20,228 out of 89,008
    • In biophysics: 705 out of 3,877
  • Year to date:
    • Site-wide: 31,467 out of 89,008
  • Since beginning of last month:
    • Site-wide: 29,366 out of 89,008

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News