tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
Daniel J. Nieves,
Hugh H. MacRae,
Matthew AB Baker,
J Justin Gooding,
Posted 10 Apr 2019
bioRxiv DOI: 10.1101/604462 (published DOI: 10.1098/rsos.191268)
Posted 10 Apr 2019
Recently, DNA-PAINT single molecule localisation microscopy (SMLM) has shown great promise for quantitative imaging. However, labelling strategies so far have relied on approaches that are multivalent or affinity-based. Here, we demonstrate tagPAINT - the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA docking strands for single molecule localisation microscopy via DNA-PAINT. We utilised tagPAINT for T-cell receptor signalling proteins at the immune synapse as a proof of principle.
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