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Inhibition of Osteoblast Proliferation and Migration by Exogenous and Endogenous Formaldehyde

By Xu Teng, Wei Huang, Hefeng Yu, Pei Wang, Weishi Li, Zhongqiang Chen, Dongwei Fan

Posted 20 Mar 2019
bioRxiv DOI: 10.1101/584052

Exogenous and endogenous formaldehyde (FA) plays an important role in cell growth and migration; however, its potential role in osteoblasts remains largely unclear. Cell counting kit-8 (CCK-8) and wound healing assays revealed that FA exposure at naturally occurring concentrations inhibited the proliferation and migration of mouse preosteoblast MC3T3-E1 cells. Moreover, RNA sequencing (RNA-seq) analysis revealed that FoxO1 signaling pathway components displayed distinct expression patterns upon FA exposure, reflected through significant enrichment of cell migration. In particular, FoxO1?Sirt1 and FA-induced related protein expression which were closely with cell proliferation and migration were confirmed by western blotting. The present results indicate that the FoxO1 pathway is involved in FA-induced inhibition of cell growth and migration.

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