Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 60,240 bioRxiv papers from 267,843 authors.
Cell type-specific monoclonal antibody cross-reactivity screening in non-human primates and development of comparative immunophenotyping panels for CyTOF
Monoclonal antibodies are a critical tool for immunologists, with applications including immunomicroscopy and cytometry, and non-human primates are essential models for drug development. Very few antibodies are raised against non-human primate antigens; instead, researchers typically use anti-human antibodies that are found to be cross-reactive with non-human primates. The NIH maintains a valuable database of cross-reactivity, but its coverage is not complete, especially for less common species. Furthermore, the database only indicates the presence or absence of staining and does not indicate if a different cell population is stained in the NHP species than in humans. We screened 332 antibodies in five immune cell populations in blood from cynomologus macaques (Macaca fascicularis), rhesus macaques (Macaca mulatta), African green monkeys (Chlorocebus aethiops) and olive/yellow baboons (Papio hamadryas anubis x Papio hamadryas cynocephalus), thereby generating a comprehensive cross-reactivity catalog that includes cell type-specificity. We subsequently used this catalog to help create large CyTOF mass cytometry panels for three of those species and humans. The curated dataset containing the primary data for each antibody has been deposited as a browsable resource at https://immuneatlas.org and https://flowrepository.org/id/FR-FCM-Z2Z7.
- Downloaded 639 times
- Download rankings, all-time:
- Site-wide: 13,355 out of 60,240
- In immunology: 220 out of 1,480
- Year to date:
- Site-wide: 2,857 out of 60,240
- Since beginning of last month:
- Site-wide: 2,272 out of 60,240
Downloads over time
Distribution of downloads per paper, site-wide
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!