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SAVE: A secure cloud-based pipeline for CRISPR pooled screen deconvolution

By Hyun-Hwan Jeong, Seon Young Kim, Maxime WC Rosseaux, Huda Y. Zoghbi, Zhandong Liu

Posted 21 Feb 2017
bioRxiv DOI: 10.1101/110262 (published DOI: 10.1093/bioinformatics/btx335)

We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, termed SAVE for Screening Analysis Visual Explorer, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, SAVE serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening datasets. Taken together, the framework described in this study is expected to accelerate development of web-based bioinformatics tool for handling all studies which include next generation sequencing data. SAVE is available at http://save.nrihub.org.

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