Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 65,106 bioRxiv papers from 288,545 authors.

HIV-1 Gag specifically restricts PI(4,5)P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly.

By Cyril Favard, Jakub Chojnacki, Peggy Merida, Naresh Yandrapalli, Johnson Mak, Christian Eggeling, Delphine Muriaux

Posted 21 Feb 2019
bioRxiv DOI: 10.1101/556308 (published DOI: 10.1126/sciadv.aaw8651)

HIV-1 Gag protein self-assembles at the plasma membrane of infected cells for viral particle formation. Gag targets lipids, mainly the phosphatidylinositol (4,5) bisphosphate, at the inner leaflet of this membrane. Here, we address the question whether Gag is able to trap specifically PI(4,5)P2 or other lipids during HIV-1 assembly in the host CD4+ T lymphocytes. Lipid dynamics within and away from HIV-1 assembly sites was determined using super-resolution STED microscopy coupled with scanning Fluorescence Correlation Spectroscopy in living T cells. Analysis of HIV-1 infected cells revealed that, upon assembly, HIV-1 is able to specifically trap PI(4,5)P2, and cholesterol, but not phosphatidylethanolamine or sphingomyelin. Furthermore, our data show that Gag is the main driving force to restrict PI(4,5)P2 and cholesterol mobility at the cell plasma membrane. This is first direct evidence showing that HIV-1 creates its own specific lipid environment by selectively recruiting PI(4,5)P2 and cholesterol, as a membrane nano-platform for virus assembly.

Download data

  • Downloaded 5,905 times
  • Download rankings, all-time:
    • Site-wide: 319 out of 65,106
    • In biophysics: 6 out of 2,744
  • Year to date:
    • Site-wide: 68 out of 65,106
  • Since beginning of last month:
    • Site-wide: 68 out of 65,106

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News