Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,521 bioRxiv papers from 264,855 authors.

Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization

By Yiming Li, Yu-Le Wu, Philipp Hoess, Markus Mund, Jonas Ries

Posted 21 Feb 2019
bioRxiv DOI: 10.1101/555730 (published DOI: 10.1364/boe.10.002708)

3D Single molecule localization microscopy relies on fitting of the individual molecules with a point spread function (PSF) model. The reconstructed images often show local squeezing or expansion in z. A common cause are depth-induced aberrations in conjunction with an imperfect PSF model calibrated from beads on a coverslip, resulting in a mismatch between measured PSF and real PSF. Here, we developed a strategy for accurate z-localization in which we use the imperfect PSF model for fitting, determine the fitting errors and correct for them in a post-processing step. We present an open-source software tool and a simple experimental calibration procedure that allow retrieving accurate z-positions in any PSF engineering approach or fitting modality, even at large imaging depths.

Download data

  • Downloaded 488 times
  • Download rankings, all-time:
    • Site-wide: 18,031 out of 57,521
    • In biophysics: 615 out of 2,431
  • Year to date:
    • Site-wide: 3,720 out of 57,521
  • Since beginning of last month:
    • Site-wide: 16,697 out of 57,521

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)