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Cell cycle dynamics of human pluripotent stem cells primed for differentiation

By Anna Shcherbina, Jingling Li, Cyndhavi Narayanan, William Greenleaf, Anshul Kundaje, Sundari Chetty

Posted 12 Feb 2019
bioRxiv DOI: 10.1101/546291 (published DOI: 10.1002/stem.3041)

Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator (FUCCI) system adapted into hPSCs and perform RNA-sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs towards differentiation.

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