Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 59,744 bioRxiv papers from 265,577 authors.
Superresolution optical fluctuation imaging (SOFI) is a simple and affordable super-resolution imaging technique and attracted a growing community over the past decade. However, the theoretical resolution enhancement of high order SOFI is still not fulfilled. In this study, we have identified "cusp artifacts" for the first time in high order SOFI images, and have shown that the high-order cumulants, odd-order moments and balanced-cumulants (bSOFI) are highly vulnerable to cusp artifacts. Our study provides guidelines for developing and screening for fluorescence probes and improving data acquisition for SOFI. The new insight is important to inspire positive utilization of the cusp artifacts.
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