Extending Chemical Perturbations Of The Ubiquitin Fitness Landscape In A Classroom Setting
Kyle A. Barlow,
Evan M. Green,
Lillian R. Kenner,
Leanna S. Morinishi,
Erin M. Poss,
Douglas R. Wassarmann,
Kurt S. Thorn,
Danielle L. Swaney,
Eric D. Chow,
Daniel N. Bolon,
Joseph L. DeRisi,
James S Fraser
Posted 17 May 2017
bioRxiv DOI: 10.1101/139352 (published DOI: 10.1242/bio.036103)
Posted 17 May 2017
Although the primary protein sequence of ubiquitin (Ub) is extremely stable over evolutionary time, it is highly tolerant to mutation during selection experiments performed in the laboratory. We have proposed that this discrepancy results from the difference between fitness under laboratory culture conditions and the selective pressures in changing environments over evolutionary time scales. Building on our previous work (Mavor et al. 2016), we used deep mutational scanning to determine how twelve new chemicals reveal novel mutational sensitivities of ubiquitin residues. We found sensitization of Lys63 in eight new conditions. In total, our experiments have uncovered a highly sensitizing condition for every position in Ub except Ser57 and Gln62. By determining the Ubiquitin fitness landscape under different chemical constraints, our work helps to resolve the inconsistencies between deep mutational scanning experiments and sequence conservation over evolutionary timescales.
- Downloaded 549 times
- Download rankings, all-time:
- Site-wide: 29,717 out of 94,912
- In bioinformatics: 3,904 out of 8,837
- Year to date:
- Site-wide: 78,619 out of 94,912
- Since beginning of last month:
- Site-wide: 80,548 out of 94,912
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!