Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain
Tiago A. Ferreira,
Michael N Economo,
Ben J. Arthur,
Donald J. Olbris,
Sean D. Murphy,
David G. Ackerman,
Bruno Dos Santos,
Joshua T. Dudmann,
Charles R. Gerfen,
Adam W Hantman,
Scott M. Sternson,
Posted 01 Feb 2019
bioRxiv DOI: 10.1101/537233 (published DOI: 10.1016/j.cell.2019.07.042)
Posted 01 Feb 2019
Neuronal cell types are the nodes of neural circuits that determine the flow of information within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides an essential descriptor of cell type and reveals how individual neurons route their output across the brain. Despite the importance of morphology, few projection neurons in the mouse brain have been reconstructed in their entirety. Here we present a robust and efficient platform for imaging and reconstructing complete neuronal morphologies, including axonal arbors that span substantial portions of the brain. We used this platform to reconstruct more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons comprise more than 75 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity.
- Downloaded 5,754 times
- Download rankings, all-time:
- Site-wide: 630 out of 94,912
- In neuroscience: 61 out of 16,862
- Year to date:
- Site-wide: 2,517 out of 94,912
- Since beginning of last month:
- Site-wide: 3,247 out of 94,912
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!