Rxivist logo

DNA methylation directs polycomb-dependent 3D genome re- organisation in naive pluripotency

By Katy A McLaughlin, Ilya M. Flyamer, John P. Thomson, Heidi K Mjoseng, Ruchi Shukla, Iain Williamson, Graeme R. Grimes, Robert S. Illingworth, Ian R. Adams, Sari Pennings, Richard R. Meehan, Wendy A Bickmore

Posted 22 Jan 2019
bioRxiv DOI: 10.1101/527309

The DNA hypomethylation that occurs when embryonic stem cells (ESCs) are directed to the ground state of naive pluripotency by culturing in 2i conditions results in redistribution of polycomb (H3K27me3) away from its target loci. Here we demonstrate that 3D genome organisation is also altered in 2i. We found chromatin decompaction at polycomb target loci as well as loss of long-range polycomb interactions. By preventing DNA hypomethylation during the transition to the ground-state, we are able to restore the H3K27me3 distribution, and polycomb-mediated 3D genome organisation that is characteristic of primed ESCs grown in serum, to ESCs in 2i. However, these cells retain the functional characteristics of 2i ground state ESCs. Our findings demonstrate the central role of DNA methylation in shaping major aspects of 3D genome organisation but caution against assuming causal roles for the epigenome and 3D genome in gene regulation and function in ESCs.

Download data

  • Downloaded 2,107 times
  • Download rankings, all-time:
    • Site-wide: 9,613
    • In cell biology: 218
  • Year to date:
    • Site-wide: 60,301
  • Since beginning of last month:
    • Site-wide: 102,676

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News