Rxivist logo

Stoichiometric quantification of spatially dense assemblies with qPAINT

By Matthew AB Baker, Daniel J. Nieves, Geva Hilzenrat, Jonathon F Berengut, Katharina Gaus, Lawrence K Lee

Posted 21 Jan 2019
bioRxiv DOI: 10.1101/525345 (published DOI: 10.1039/C9NR00472F)

Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.

Download data

  • Downloaded 394 times
  • Download rankings, all-time:
    • Site-wide: 40,938 out of 89,005
    • In biophysics: 1,632 out of 3,876
  • Year to date:
    • Site-wide: 79,443 out of 89,005
  • Since beginning of last month:
    • Site-wide: 35,905 out of 89,005

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)