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Stoichiometric quantification of spatially dense assemblies with qPAINT

By Matthew AB Baker, Daniel J. Nieves, Geva Hilzenrat, Jonathon F Berengut, Katharina Gaus, Lawrence K Lee

Posted 21 Jan 2019
bioRxiv DOI: 10.1101/525345 (published DOI: 10.1039/C9NR00472F)

Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.

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