Rxivist logo

The KZFP/KAP1 system controls transposable elements-embedded regulatory sequences in adult T cells

By Flavia Marzetta, Laia Simó-Riudalbas, Julien Duc, Evarist Planet, Sonia Verp, Priscilla Turelli, Didier Trono

Posted 19 Jan 2019
bioRxiv DOI: 10.1101/523597

Transposable elements-embedded regulatory sequences (TEeRS) are subjected to early embryonic repression through sequence-specific recruitment of KRAB zinc finger proteins (KZFPs), their cofactor KAP1/TRIM28 and associated chromatin modifiers. This modulates the TEeRS-mediated regulation of gene expression in embryonic stem cells (ESCs) and leads to DNA methylation-induced silencing. However, KZFPs are broadly expressed in adult tissues, suggesting that they control TEeRS throughout life. Confirming this hypothesis, we reveal here that the KZFP/KAP1 system exerts a highly dynamic control of TEeRS in adult human CD4+ T lymphocytes. First, we observed that in these cells many TEs are still bound by KAP1, the recruitment of which is dynamically regulated upon T cell receptor stimulation. Second, we found that KAP1 depletion induces broad transcriptional alterations in T cells, with de-repression of TE-based regulatory elements leading to the illegitimate activation of nearby genes. Finally, we show that the tissue-restricted expression of KZFPs correlates with KAP1-mediated lineage-specific chromatin signatures and transcriptional repression. These data support a model where TE-targeting KZFPs and KAP1 are important regulators of gene expression in adult human cells.

Download data

  • Downloaded 1,390 times
  • Download rankings, all-time:
    • Site-wide: 17,382
    • In genomics: 1,660
  • Year to date:
    • Site-wide: 28,871
  • Since beginning of last month:
    • Site-wide: 25,878

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News