Rxivist logo

Structural basis for functional interactions in dimers of SLC26 transporters

By Yung-Ning Chang, Eva A Jaumann, Katrin Reichel, Julia Hartmann, Dominik Oliver, Gerhard Hummer, Benesh Joseph, Eric R. Geertsma

Posted 11 Jan 2019
bioRxiv DOI: 10.1101/518209 (published DOI: 10.1038/s41467-019-10001-w)

The SLC26 family of transporters maintains anion equilibria in all kingdoms of life. The family shares a 7 + 7 transmembrane segments inverted repeat architecture with the SLC4 and SLC23 families, but holds a regulatory STAS domain in addition. While the only experimental SLC26 structure is monomeric, SLC26 proteins form structural and functional dimers in the lipid membrane. Here we resolve the structure of an SLC26 dimer embedded in a lipid membrane and characterize its functional relevance by combining PELDOR distance measurements and biochemical studies with MD simulations and spin-label ensemble refinement. Our structural model reveals a unique interface different from the SLC4 and SLC23 families. The functionally relevant STAS domain exerts a stabilizing effect on regions central in this dimer. Characterization of heterodimers indicates that protomers in the dimer functionally interact. The combined structural and functional data define the framework for a mechanistic understanding of functional cooperativity in SLC26 dimers.

Download data

  • Downloaded 713 times
  • Download rankings, all-time:
    • Site-wide: 43,270
    • In biochemistry: 1,094
  • Year to date:
    • Site-wide: 117,382
  • Since beginning of last month:
    • Site-wide: 142,872

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide