Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,118 bioRxiv papers from 295,271 authors.

Direct capture of CRISPR guides enables scalable, multiplexed, and multi-omic Perturb-seq

By Joseph M Replogle, Albert Xu, Thomas M Norman, Elliott J Meer, Jessica M Terry, Daniel Riordan, Niranjan Srinivas, Tarjei S. Mikkelsen, Jonathan S Weissman, Britt Adamson

Posted 21 Dec 2018
bioRxiv DOI: 10.1101/503367

Pairing CRISPR-based genetic screens with single-cell transcriptional phenotypes (Perturb-seq) has advanced efforts to explore the function of mammalian genes and genetic networks. We present strategies for Perturb-seq that enable direct capture of CRISPR sgRNAs within 3' or 5' single-cell RNA-sequencing libraries using the 10x Genomics platform. This technology greatly expands the accessibility, scalability, and flexibility of Perturb-seq, specifically enabling use with programmed combinatorial perturbations and multiplexing with multi-omic measurements.

Download data

  • Downloaded 4,558 times
  • Download rankings, all-time:
    • Site-wide: 525 out of 67,121
    • In genomics: 137 out of 4,561
  • Year to date:
    • Site-wide: 131 out of 67,121
  • Since beginning of last month:
    • Site-wide: 379 out of 67,121

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)