Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,118 bioRxiv papers from 295,271 authors.
Direct capture of CRISPR guides enables scalable, multiplexed, and multi-omic Perturb-seq
Joseph M Replogle,
Thomas M Norman,
Elliott J Meer,
Jessica M Terry,
Tarjei S. Mikkelsen,
Jonathan S Weissman,
Posted 21 Dec 2018
bioRxiv DOI: 10.1101/503367
Posted 21 Dec 2018
Pairing CRISPR-based genetic screens with single-cell transcriptional phenotypes (Perturb-seq) has advanced efforts to explore the function of mammalian genes and genetic networks. We present strategies for Perturb-seq that enable direct capture of CRISPR sgRNAs within 3' or 5' single-cell RNA-sequencing libraries using the 10x Genomics platform. This technology greatly expands the accessibility, scalability, and flexibility of Perturb-seq, specifically enabling use with programmed combinatorial perturbations and multiplexing with multi-omic measurements.
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