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BUSCO applications from quality assessments to gene prediction and phylogenomics

By Robert Michael Waterhouse, Mathieu Seppey, Felipe Aristides Simao Neto, Mosè Manni, Panagiotis Ioannidis, Guennadi Klioutchnikov, Evgenia V. Kriventseva, Evgeny M Zdobnov

Posted 17 Aug 2017
bioRxiv DOI: 10.1101/177485 (published DOI: 10.1093/molbev/msx319)

Genomics promises comprehensive surveying of genomes and metagenomes, but rapidly changing technologies and expanding data volumes make evaluation of completeness a challenging task. Technical sequencing quality metrics can be complemented by quantifying completeness in terms of the expected gene content of Benchmarking Universal Single-Copy Orthologs (BUSCO, http://busco.ezlab.org). Now in its third release, BUSCO utilities extend beyond quality control to applications in comparative genomics, gene predictor training, metagenomics, and phylogenomics.

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