Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 62,232 bioRxiv papers from 276,305 authors.

Single-cell analysis of Crohn's disease lesions identifies a pathogenic cellular module associated with resistance to anti TNF therapy

By Jerome Christophe Martin, G Boschetti, C Chang, R Ungaro, M Giri, LS Chuang, S Nayar, A Greenstein, M. Dubinsky, L Walker, A Leader, JS Fine, CE Whitehurst, L Mbow, S Kugathasan, L.A. Denson, J. Hyams, JR Friedman, P Desai, HM Ko, I Laface, Guray Akturk, EE Schadt, S Gnjatic, A. Rahman, M Merad, JH Cho, E Kenigsberg

Posted 20 Dec 2018
bioRxiv DOI: 10.1101/503102 (published DOI: 10.1016/j.cell.2019.08.008)

Clinical benefits to cytokine blockade in ill Crohn's disease (iCD) have been limited to a subset of patients. Whether cellular and molecular heterogeneity contributes to variability in treatment responses has been unclear. Using single cell technologies combining scRNAseq, CyTOF and multiplex tissue imaging, we mapped the cellular landscape of inflamed ileum lesions, adjacent non-inflamed ileum and matched circulating blood cells of iCD patients. In inflamed tissues, we identified a pathogenic module characterized by an inflammatory mononuclear phagocyte (Inf.MNP)-associated cellular response organized around inflammatory macrophages and mature dendritic cells in a subset of iCD patients. We confirmed the Inf.MNP-associated cellular response in 4 independent iCD cohorts (n=441) and showed that presence of this pathogenic module at diagnosis correlated with primary resistance to anti-TNF therapy. Single cell mapping of iCD tissues identifies a complex cellular signature of anti-TNF resistance thereby revealing novel biomarkers of treatment response and tailored therapeutic opportunities.

Download data

  • Downloaded 2,747 times
  • Download rankings, all-time:
    • Site-wide: 1,254 out of 62,253
    • In immunology: 16 out of 1,545
  • Year to date:
    • Site-wide: 319 out of 62,253
  • Since beginning of last month:
    • Site-wide: None out of 62,253

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News