Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,524 bioRxiv papers from 264,866 authors.

The neuropeptide Galanin is required for homeostatic rebound sleep following increased neuronal activity

By Sabine Reichert, Oriol Pavon Arocas, Jason Rihel

Posted 27 Nov 2018
bioRxiv DOI: 10.1101/479634

Sleep pressure homeostatically increases during wake and dissipates during sleep, but the molecular signals and neuronal substrates that measure homeostatic sleep pressure remain poorly understood. We present a pharmacological assay in larval zebrafish that generates acute, short-term increases in wakefulness followed by sustained rebound sleep after washout. The intensity of global neuronal activity during drug-induced wakefulness predicted the amount of subsequent rebound sleep. Whole brain mapping with the neuronal activity marker phosphorylated extracellular signal regulated kinase (pERK) identified preoptic Galanin expressing neurons as selectively active during rebound sleep, and the relative induction of galanin transcripts was predictive of total rebound sleep time. Galanin is required for sleep homeostasis, as galanin mutants almost completely lacked rebound sleep following both pharmacologically induced neuronal activity and physical sleep deprivation. These results suggest that Galanin plays a key role in responding to sleep pressure signals derived from neuronal activity and functions as an output arm of the vertebrate sleep homeostat.

Download data

  • Downloaded 545 times
  • Download rankings, all-time:
    • Site-wide: 15,708 out of 57,524
    • In neuroscience: 2,569 out of 10,117
  • Year to date:
    • Site-wide: 4,713 out of 57,524
  • Since beginning of last month:
    • Site-wide: 7,532 out of 57,524

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)