A Primase-Induced Conformational Switch Controls the Stability of the Bacterial Replisome
Jacob S. Lewis,
Allen T.Y. Lo,
Valerie L. O’Shea,
James M. Berger,
Nicholas E Dixon,
Antoine M van Oijen
Posted 13 Nov 2018
bioRxiv DOI: 10.1101/469312 (published DOI: 10.1016/j.molcel.2020.04.037)
Posted 13 Nov 2018
Recent studies of bacterial DNA replication have led to a picture of the replisome as an entity that freely exchanges DNA polymerases and displays intermittent coupling between the helicase and polymerase(s). Challenging the textbook model of the polymerase holoenzyme acting as a stable complex coordinating the replisome, these observations suggest a role of the helicase as the central organizing hub. We show here that the molecular origin of this newly-found plasticity lies in the >400-fold increase in strength of the interaction between the polymerase holoenzyme and the replicative helicase upon association of the primase with the replisome. By combining in vitro ensemble-averaged and single-molecule assays, we demonstrate that this conformational switch operates during replication and promotes recruitment of multiple holoenzymes at the fork. Our observations provide a molecular mechanism for polymerase exchange and offer a revised model for the replication reaction that emphasizes its stochasticity.
- Downloaded 382 times
- Download rankings, all-time:
- Site-wide: 44,130 out of 92,738
- In biochemistry: 1,312 out of 3,116
- Year to date:
- Site-wide: 52,050 out of 92,738
- Since beginning of last month:
- Site-wide: 35,467 out of 92,738
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!