Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,506 bioRxiv papers from 264,779 authors.

A stable, high refractive index, switching buffer for super-resolution imaging

By Tobias MP Hartwich, Kenny KH Chung, Lena Schroeder, Joerg Bewersdorf, Christian Soeller, David Baddeley

Posted 08 Nov 2018
bioRxiv DOI: 10.1101/465492

dSTORM super-resolution imaging relies on switching buffers to enable dye molecules to enter and exit a metastable dark state. Current buffers have a very limited shelf life of approximately 1 day and poorly match sample refractive index, impacting negatively on measurement reproducibility and image fidelity. We present a buffer based on chemical, rather than enzymatic, oxygen scavenging which exhibits dramatically improved stability, switching speed, contrast, and index matching.

Download data

  • Downloaded 1,087 times
  • Download rankings, all-time:
    • Site-wide: 5,631 out of 57,506
    • In biophysics: 149 out of 2,431
  • Year to date:
    • Site-wide: 4,742 out of 57,506
  • Since beginning of last month:
    • Site-wide: 7,901 out of 57,506

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)