Habitat spatial structure has a profound influence on bacterial life, yet there currently are no low-cost equipment-free laboratory techniques to reproduce the intricate structure of natural bacterial habitats. Here, we demonstrate the use of paper scaffolds to create landscapes spatially structured at the scales relevant to bacterial ecology. In paper scaffolds, planktonic bacteria migrate through liquid filled pores, while the paper's cellulose fibers serve as anchor points for sessile colonies (biofilms). Using this novel approach we explore bacterial colonization dynamics in different landscape topographies, and characterize the community composition of Escherichia coli strains undergoing centimeter-scale range expansions in habitats structured at the micrometer scale. The bacteria-in-paper platform enables quantitative assessment of bacterial community dynamics in complex environments using everyday materials.
- Downloaded 975 times
- Download rankings, all-time:
- Site-wide: 12,399 out of 92,509
- In ecology: 288 out of 4,078
- Year to date:
- Site-wide: 13,422 out of 92,509
- Since beginning of last month:
- Site-wide: 14,570 out of 92,509
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!